Coghlan J P, Fan J S, Scoggins B A, Shulkes A A
Aust J Biol Sci. 1977 Apr;30(1-2):71-84.
Methods are described for the simultaneous measurement of extracellular fluid volume (ECFV) and plasma volume (PV) in sheep using dilution of 82Br (as sodium bromide) and 131I-labelled ovine gamma globulin. Following injection of 82Br (100 micronCi), equilibrium in blood was reached after 3 h at which time only 4% of the injected dose was in rumen water. The ECFV was measured as the mean of the 2- and 3-h bromide space after correction for the relative water content of plasma, the Gibbs-Donnan factor and the loss of 82Br into red blood cells. 131I-labelled ovine gamma globulin (20 micronCi) was injected after the 3-h 82 Br space was obtained and blood samples were taken at 10, 20, 30 and 40 min. In 16 determinations in 11 sheep (25-47 kg body weight) the mean (+/- s.e.m.) ECFV was 9112 +/- 289 ml (or 245 +/- 9 ml/kg). The mean PV for 16 observations in 11 sheep measured together with ECFV was 1597 +/- 62 ml (or 42-8 +/- 1-8 ml/kg). Although there was no relationship between body weight and PV there was a significant correlation between ECFV and body weight and also significant negative correlations between body weight and ECFV or PV when these were expressed as a function of body weight. The variation in ECFV measured on four occasions over 7-10 days in four sheep was 3-5% (range 2-6-4-6%). For PV measured in two animals on two consecutive days at the same time as ECFV the coefficient of variation was 1-5 and 2-1%. Acute sodium depletion (250-670 mmol) by parotid duct cannulation in three sheep resulted in a fall in ECFV which would account for only 15-20% of the sodium deficit. The remainder is presumably derived from ruminal sodium stores.
本文描述了一种利用82Br（以溴化钠形式）和131I标记的绵羊γ球蛋白稀释法同时测量绵羊细胞外液量（ECFV）和血浆量（PV）的方法。注射82Br（100微居里）后，3小时后血液达到平衡，此时注入剂量中只有4%存在于瘤胃液中。ECFV通过校正血浆相对含水量、吉布斯-唐南因子以及82Br进入红细胞的损失后，以2小时和3小时溴化物空间的平均值来测量。在获得3小时82Br空间后，注射131I标记的绵羊γ球蛋白（20微居里），并在10、20、30和40分钟采集血样。在11只绵羊（体重25 - 47千克）的16次测定中，平均（±标准误）ECFV为9112 ± 289毫升（或245 ± 9毫升/千克）。与ECFV一起测量的11只绵羊的16次观察中，平均PV为1597 ± 62毫升（或42.8 ± 1.8毫升/千克）。虽然体重与PV之间没有关系，但ECFV与体重之间存在显著相关性，并且当ECFV或PV表示为体重的函数时，体重与ECFV或PV之间也存在显著负相关。在4只绵羊中，7 - 10天内四次测量的ECFV变化为3.5%（范围2.6 - 4.6%）。对于与ECFV同时在两只动物上连续两天测量的PV，变异系数分别为1.5%和2.1%。三只绵羊通过腮腺导管插管进行急性钠耗竭（250 - 670毫摩尔）导致ECFV下降，而这仅占钠缺乏量的15 - 20%。其余部分可能来自瘤胃钠储备。
