Etcheverry S B, Crans D C, Keramidas A D, Cortizo A M
Catedra de Bioquimica Patologica, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Arch Biochem Biophys. 1997 Feb 1;338(1):7-14. doi: 10.1006/abbi.1996.9778.
Vanadium compounds mimic insulin actions in different cell types. The present study concerns the insulin-like effects of three vanadium(V) derivatives and one vanadium(IV) complex on osteoblast-like (UMR106 and MC3T3E1) cells in culture. The vanadium oxalate and vanadium citrate complexes hydrolyzed completely under the culture conditions, whereas more than 40% of the vanadium tartrate and nitrilotriacetate complexes remained. Vanadate, as well as vanadium oxalate, citrate, and tartrate complexes enhanced cell proliferation (as measured by the crystal violet assay), glucose consumption, and protein content in UMR106 and MC3T3E1 osteoblast-like cells. The vanadium nitrilotriacetate complex (the only peroxo complex tested) stimulated cell proliferation in UMR106 but not in MC3T3E1 cells. This derivative strongly transformed the morphology of the MC3T3E1 cells. All vanadium(V) compounds inhibited cell differentiation (alkaline phosphatase activity) in UMR106 cells. Our data are consistent with the interpretation that vanadium oxalate and citrate complexes hydrolyze to vanadate. Vanadium nitrilotriacetate would appear to be toxic for normal MC3T3E1 osteoblasts. In contrast, the vanadium tartrate complex induced a proliferative effect; however, it did not alter cell differentiation.
钒化合物在不同细胞类型中模拟胰岛素的作用。本研究关注三种钒（V）衍生物和一种钒（IV）配合物对培养的成骨样细胞（UMR106和MC3T3E1）的胰岛素样作用。草酸钒和柠檬酸钒配合物在培养条件下完全水解，而酒石酸钒和次氮基三乙酸钒配合物仍有超过40%残留。钒酸盐以及草酸钒、柠檬酸钒和酒石酸钒配合物可增强UMR106和MC3T3E1成骨样细胞的增殖（通过结晶紫测定法测量）、葡萄糖消耗和蛋白质含量。次氮基三乙酸钒配合物（唯一测试的过氧配合物）刺激UMR106细胞增殖，但对MC3T3E1细胞无此作用。该衍生物强烈改变了MC3T3E1细胞的形态。所有钒（V）化合物均抑制UMR106细胞的分化（碱性磷酸酶活性）。我们的数据与草酸钒和柠檬酸钒配合物水解为钒酸盐的解释一致。次氮基三乙酸钒似乎对正常的MC3T3E1成骨细胞有毒性。相比之下，酒石酸钒配合物诱导了增殖作用；然而，它并未改变细胞分化。
