Jurgens G, Lindström K, Saano A
Department of Applied Chemistry and Microbiology, Helsinki University, Finland.
Appl Environ Microbiol. 1997 Feb;63(2):803-5. doi: 10.1128/aem.63.2.803-805.1997.
We report here the results of phylogenetic analysis of archaeal 16S rRNA gene sequences amplified by PCR with Archaea-specific primers with mixed-population DNA extracted directly from forest soil used as a template. Nucleotide signature and phylogenetic analyses show that the sequences obtained belong to the domain Archaea and form a new cluster. Its phylogenetic position suggests that sequences are from a previously undescribed terrestrial group within the kingdom Crenarchaeota.
我们在此报告,以直接从森林土壤中提取的混合种群DNA为模板,用古菌特异性引物通过PCR扩增古菌16S rRNA基因序列的系统发育分析结果。核苷酸特征和系统发育分析表明,所获得的序列属于古菌域,并形成一个新的簇。其系统发育位置表明,这些序列来自泉古菌门内一个以前未描述的陆地类群。