Aleshkin G I, Efanova E A, Komissarova L V, Kozlovskiĭ V N, Rozhkov E V, Zakharenko V I
Zh Mikrobiol Epidemiol Immunobiol. 1996 Jul-Aug(4):18-22.
Monoclonal antibodies to F.tularensis cells, subspecies holarctica, were studied for the capacity of reacting with F.tularensis, subspecies nearctica, and its mutants having lower virulence and altered capacity for inducing protective immunity to tularemia in laboratory animals. Among the antibody-producing hybridoma clones under study, clones F8/67 and C7/65 capable of distinguishing the mutants of F.tularensis, subspecies nearctica, with lower virulence than that of the initial strain were selected. Antibodies of these hybridoma clones did not interact with the antigens of the initial virulent strains of F.tularensis, subspecies nearctica, while giving pronounced reaction with the antigens of its mutants. Close F8/67 produced IgG antibodies and clone C7/65, IgM antibodies. As shown in immunoblotting, antibodies produced by these hybridoma clones bound with proteins of F.tularensis cell membranes.
对针对土拉热弗朗西斯菌全北区亚种细胞的单克隆抗体进行了研究，以考察其与土拉热弗朗西斯菌近北区亚种及其毒力较低且诱导实验动物对兔热病产生保护性免疫能力改变的突变体发生反应的能力。在所研究的产生抗体的杂交瘤克隆中，选择了能够区分出土拉热弗朗西斯菌近北区亚种中比初始菌株毒力更低的突变体的克隆F8/67和C7/65。这些杂交瘤克隆的抗体不与土拉热弗朗西斯菌近北区亚种初始强毒株的抗原发生相互作用，而与其突变体的抗原发生明显反应。克隆F8/67产生IgG抗体，克隆C7/65产生IgM抗体。如免疫印迹所示，这些杂交瘤克隆产生的抗体与土拉热弗朗西斯菌细胞膜蛋白结合。
