Pontón J, Bikandi J, Moragues M D, Arilla M C, Elósegui R, Quindós G, Fisicaro P, Conti S, Polonelli L
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicine y Odontología, Universidad del País Vasco, Bilbao, Vizcaya, Spain.
J Dent Res. 1996 Dec;75(12):1979-85. doi: 10.1177/00220345960750121001.
Salivary secretory IgA (sIgA) has been shown to react with a group of heat shock mannoproteins preferentially expressed on yeast cells grown at 37 degrees C. Since at this temperature C. albicans can induce germ tubes, we explored the role of germ tube induction on human salivary sIgA reactivity in both germinative and agerminative C. albicans strains, in an attempt to investigate whether the germ tube expressed the heat shock mannoproteins reactive with sIgA. The reactivity with sIgA of the agerminative strain, grown at 25 and 37 degrees C for different times, was measured spectrofluorometrically and was fairly constant with time. Yeast cells grown at 37 degrees C tended to be more reactive than those grown at 25 degrees C. In contrast, when compared with the yeast cells of the germinative strain grown at 25 degrees C, there was a statistically significant decrease in reactivity with sIgA during germ tube formation. Serum IgA and IgG did not show statistically significant changes in reactivity with C. albicans during germination, suggesting differences in reactivity with C. albicans cell wall antigens between mucosal and systemic humoral responses. Cell wall mannoproteins of molecular masses > 60 kDa were characterized by Western blotting as responsible for the decrease in sIgA reactivity observed in the germ tube, and the fall in sIgA reactivity was related to the release of cell wall mannoproteins into the culture medium. The release of these mannoproteins may be a mechanism whereby C. albicans avoids the action of sIgA, and it may play an important role in the post-parasite relationship in oral candidiasis.
唾液分泌型 IgA（sIgA）已被证明可与一组在 37 摄氏度生长的酵母细胞上优先表达的热休克甘露糖蛋白发生反应。由于在这个温度下白色念珠菌可诱导芽管形成，我们探讨了芽管诱导在有芽管形成和无芽管形成的白色念珠菌菌株中对人唾液 sIgA 反应性的作用，试图研究芽管是否表达了与 sIgA 反应的热休克甘露糖蛋白。用荧光分光光度法测定了在 25 摄氏度和 37 摄氏度下培养不同时间的无芽管形成菌株与 sIgA 的反应性，其反应性随时间相当稳定。在 37 摄氏度生长的酵母细胞往往比在 25 摄氏度生长的酵母细胞反应性更强。相比之下，与在 25 摄氏度生长的有芽管形成菌株的酵母细胞相比，在芽管形成过程中与 sIgA 的反应性有统计学上的显著下降。血清 IgA 和 IgG 在芽管形成过程中与白色念珠菌的反应性没有统计学上的显著变化，这表明黏膜和全身体液反应与白色念珠菌细胞壁抗原的反应性存在差异。通过蛋白质印迹法鉴定出分子量 > 60 kDa 的细胞壁甘露糖蛋白是芽管中观察到的 sIgA 反应性降低的原因，sIgA 反应性的下降与细胞壁甘露糖蛋白释放到培养基中有关。这些甘露糖蛋白的释放可能是白色念珠菌逃避 sIgA 作用的一种机制，并且它可能在口腔念珠菌病的寄生虫后关系中起重要作用。
