Polonelli L, Gerloni M, Conti S, Fisicaro P, Cantelli C, Portincasa P, Almondo F, Barea P L, Hernando F L, Ponton J
Istituto di Microbiologia, Università degli Studi di Parma, Italy.
J Infect Dis. 1994 Jun;169(6):1401-5. doi: 10.1093/infdis/169.6.1401.
The expression of Candida albicans antigenic determinants reacting with secretory IgA from patients with oral and vaginal candidiasis was investigated under different in vitro conditions. Reversible antigenic transitions were inducible in synthetic medium by temperature shifts, as the yeast cells were positive by an indirect immunofluorescence assay after being incubated at 37 degrees C but not at 25 degrees C. In vitro temperature-inducible C. albicans antigenic determinants reactive with secretory IgA were characterized by radioimmune Western blot as mannoproteins with molecular masses of 180-200, 130-150, 90-110, and 60-70 kDa. This is the first report on the expression of mannoproteins regulated by temperature involved in the secretory immune response during mucosal candidiasis.
在不同体外条件下，研究了白色念珠菌抗原决定簇与口腔和阴道念珠菌病患者分泌型IgA的反应情况。在合成培养基中，通过温度变化可诱导白色念珠菌发生可逆的抗原转变，因为酵母细胞在37℃孵育后经间接免疫荧光试验呈阳性，而在25℃孵育则不然。通过放射免疫蛋白质印迹法鉴定，与分泌型IgA反应的体外温度诱导型白色念珠菌抗原决定簇为分子量180 - 200、130 - 150、90 - 110和60 - 70 kDa的甘露糖蛋白。这是关于黏膜念珠菌病期间参与分泌免疫反应的温度调节甘露糖蛋白表达的首次报道。
