Bischoff S C, Grabowsky J, Manns M P
Department of Gastroenterology and Hepatology, Medical School of Hannover, Germany.
Dig Dis Sci. 1997 Feb;42(2):394-403. doi: 10.1023/a:1018886423475.
Previous studies indicated that eosinophils and mast cells accumulate and become activated in inflammatory bowel disorders. The aim of the study was to examine the presence of eosinophil and mast cell mediators in human stool samples of patients with inflammatory bowel disorders. We measured eosinophil cationic protein (ECP), eosinophil protein X (EPX), methylhistamine, and alpha 1-antitrypsin in fecal samples of 136 patients (62 Crohn's disease, 24 ulcerative colitis, 15 intestinal food allergy, 35 other gastrointestinal diseases) and 8 healthy controls. We found strongly elevated levels of ECP (median: 29, range: 0.4-1783 ng/g feces) and EPX (803, 10-33,225 ng/g) in all patients groups compared to controls (ECP: 1.5, 0.5-55 ng/g; EPX: 235, 12-746 ng/g). Similar results, albeit less pronounced, were obtained for methylhistamine and alpha 1-antitrypsin. Particularly high concentrations of ECP and EPX were found in patients with active mucosal inflammation. In conclusion, the study presents an easy and reliable method for the detection of fecal ECP, EPX, and methylhistamine and may provide a tool to gain insight into the pathogenesis of inflammatory bowel diseases and have potential as diagnostic test.
先前的研究表明，嗜酸性粒细胞和肥大细胞在炎症性肠病中会积聚并被激活。本研究的目的是检测炎症性肠病患者粪便样本中嗜酸性粒细胞和肥大细胞介质的存在情况。我们测量了136例患者（62例克罗恩病、24例溃疡性结肠炎、15例肠道食物过敏、35例其他胃肠道疾病）以及8名健康对照者粪便样本中的嗜酸性粒细胞阳离子蛋白（ECP）、嗜酸性粒细胞蛋白X（EPX）、甲基组胺和α1-抗胰蛋白酶。我们发现，与对照组相比（ECP：1.5，0.5 - 55 ng/g；EPX：235，12 - 746 ng/g），所有患者组的ECP（中位数：29，范围：0.4 - 1783 ng/g粪便）和EPX（803，10 - 33,225 ng/g）水平均显著升高。甲基组胺和α1-抗胰蛋白酶也得到了类似的结果，尽管不太明显。在有活动性黏膜炎症的患者中发现了特别高浓度的ECP和EPX。总之，该研究提出了一种检测粪便ECP、EPX和甲基组胺的简便可靠方法，可能为深入了解炎症性肠病的发病机制提供一种工具，并具有作为诊断测试的潜力。
