Mizuhira V, Hasegawa H
Medical Research Institute, Tokyo Medical and Dental University, Japan.
Eur J Morphol. 1996 Dec;34(5):385-91. doi: 10.1076/ejom.34.5.385.13055.
More than a decade ago, we introduced a microprocessed household microwave oven for microwave-stimulated fixation. In this paper we present an overview of our experience. We have introduced the use of tannic acid (TA, 0.1%), which is very effective for fixing soluble peptides or proteins, except in the case of enzymo-cytochemistry. In the latter case, a combination of TA with aldehyde in the microwave-fixation step brings perfect fixation, but inactivation of the enzymes and their enzymatic activities.
十多年前，我们推出了一款用于微波刺激固定的微处理家用微波炉。在本文中，我们概述了我们的经验。我们引入了单宁酸（TA，0.1%）的使用，它对固定可溶性肽或蛋白质非常有效，但酶细胞化学情况除外。在后一种情况下，在微波固定步骤中将TA与醛结合可实现完美固定，但会使酶及其酶活性失活。
