通过直接电场控制快速测定DNA杂交体中的单碱基错配突变
Rapid determination of single base mismatch mutations in DNA hybrids by direct electric field control.
作者信息
Sosnowski R G, Tu E, Butler W F, O'Connell J P, Heller M J
机构信息
Nanogen Inc., San Diego, CA 92121, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1119-23. doi: 10.1073/pnas.94.4.1119.
Abstract
We have demonstrated that controlled electric fields can be used to regulate transport, concentration, hybridization, and denaturation of single- and double-stranded oligonucleotides. Discrimination among oligonucleotide hybrids with widely varying binding strengths may be attained by simple adjustment of the electric field strength. When this approach is used, electric field denaturation control allows single base pair mismatch discrimination to be carried out rapidly (<15 sec) and with high resolution. Electric field denaturation takes place at temperatures well below the melting point of the hybrids, and it may constitute a novel mechanism of DNA denaturation.
摘要
我们已经证明，可控电场可用于调节单链和双链寡核苷酸的转运、浓度、杂交和变性。通过简单调整电场强度，可以实现对结合强度差异很大的寡核苷酸杂交体的区分。使用这种方法时，电场变性控制能够快速（<15秒）且高分辨率地进行单碱基对错配区分。电场变性在远低于杂交体熔点的温度下发生，它可能构成一种DNA变性的新机制。
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