Goffeau A, Park J, Paulsen I T, Jonniaux J L, Dinh T, Mordant P, Saier M H
Unité de Biochimie Physiologique, Université de Louvain, Louvain-la-Neuve, Belgium.
Yeast. 1997 Jan;13(1):43-54. doi: 10.1002/(SICI)1097-0061(199701)13:1<43::AID-YEA56>3.0.CO;2-J.
Screening of the complete genome sequence from the yeast Saccharomyces cerevisiae reveals that 28 open reading frames (ORFs) are homologous to each other and to established bacterial members of the drug-resistant subfamily of the major facilitator superfamily. The phylogenesis of these protein sequences shows that they fall into three major clusters. Cluster I contains 12 ORFs, cluster II contains ten ORFs and cluster III contains six ORFs. Hydropathy analyses indicate that in cluster II and III ORFs, 14 transmembrane spans are predicted whereas only 12 transmembrane spans are predicted in cluster I ORFs. Three ORFs that have known functions as multidrug-resistance pumps in other yeast species such as Schizosaccharomyces pombe (CAR1), Candida albicans (BMRP) or C. maltosa (CYHR), also fall into cluster I. Two S. cerevisiae ORFs of known multidrug-resistance function (ATR1, SGE1) fall into cluster II. Cluster III consists exclusively of ORFs of unknown function but binary sequence comparisons show homology to ORFs from cluster II. Analysis of the multiple alignment for these proteins leads to the identification of characteristic signature sequences for each of the three clusters.
对酿酒酵母完整基因组序列的筛选显示，有28个开放阅读框（ORF）彼此同源，并且与主要易化子超家族耐药亚家族的已知细菌成员同源。这些蛋白质序列的系统发生分析表明，它们分为三个主要簇。簇I包含12个ORF，簇II包含10个ORF，簇III包含6个ORF。亲水性分析表明，在簇II和III的ORF中，预测有14个跨膜区，而在簇I的ORF中仅预测有12个跨膜区。在其他酵母物种如粟酒裂殖酵母（CAR1）、白色念珠菌（BMRP）或麦芽糖假丝酵母（CYHR）中具有已知多药耐药泵功能的三个ORF也属于簇I。已知具有多药耐药功能的两个酿酒酵母ORF（ATR1、SGE1）属于簇II。簇III仅由功能未知的ORF组成，但二元序列比较显示与簇II的ORF同源。对这些蛋白质的多重比对分析导致鉴定出三个簇各自的特征性签名序列。
