Van Dyck L, Tettelin H, Purnelle B, Goffeau A
Unité de Biochimie Physiologique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
Yeast. 1997 Feb;13(2):171-6. doi: 10.1002/(SICI)1097-0061(199702)13:2<171::AID-YEA57>3.0.CO;2-V.
An 18.3 kb DNA segment from yeast Saccharomyces cerevisiae VII encompasses the previously characterized MEP1, NUP57 and PPT1 genes as well as seven new open reading frames (ORFs) of at least 100 residues. G6358 is an ubiquitous glutamine-dependent asparagine synthase. G6362 is membrane protein highly homologous to a protein of unknown function in the yeast Schizosaccharomyces pombe. Three ORFs (G6324, G6335 and G6365) have no significant homology with previously reported proteins of characteristic motifs. G6321 and G6359, enclosed in longer ORFs, are not likely to be coding. The segment also contains tRNA genes for Asn, Arg and Ile as well as sigma element and two solo deltas. ORFs and genetic elements are named according to a preliminary working nomenclature.
来自酿酒酵母VII的一个18.3 kb DNA片段包含先前已鉴定的MEP1、NUP57和PPT1基因，以及7个新的至少含100个残基的开放阅读框（ORF）。G6358是一种普遍存在的谷氨酰胺依赖性天冬酰胺合成酶。G6362是一种膜蛋白，与粟酒裂殖酵母中一种功能未知的蛋白高度同源。三个ORF（G6324、G6335和G6365）与先前报道的具有特征基序的蛋白没有明显同源性。包含在较长ORF中的G6321和G6359不太可能编码。该片段还包含Asn、Arg和Ile的tRNA基因，以及sigma元件和两个单独的δ元件。ORF和遗传元件根据初步工作命名法命名。
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