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对来自七号染色体左臂的21.7kb DNA序列进行分析，发现了11个开放阅读框：其中两个对应新基因。

Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes.

作者信息

Feuermann M, Simeonova L, Souciet J L, Potier S

机构信息

Laboratoire de Microbiologie et Génétique, URA 1481 Université Louis Pasteur/CNRS, Strasbourg, France.

出版信息

Yeast. 1997 Apr;13(5):475-7. doi: 10.1002/(SICI)1097-0061(199704)13:5<475::AID-YEA101>3.0.CO;2-0.

DOI:10.1002/(SICI)1097-0061(199704)13:5<475::AID-YEA101>3.0.CO;2-0
PMID:9153757
Abstract

The DNA sequence of a fragment of 21731 bp (nucleotides 87408 to 109138) located on the left arm of chromosome VII from Saccharomyces cerevisiae S288C has been determined using a random cloning strategy followed by an oligonucleotide-directed sequencing. This fragment contains eight complete genes previously sequenced (CLG1, SKI8, VAM7, YPT32, MIG2, SIP2, SPT16 and CHC1), the 5' part of POX1 and two other complete unidentified open reading frames of more than 100 amino acids.

摘要

利用随机克隆策略并结合寡核苷酸定向测序，已确定了酿酒酵母S288C七号染色体左臂上一段21731 bp（核苷酸87408至109138）片段的DNA序列。该片段包含八个先前已测序的完整基因（CLG1、SKI8、VAM7、YPT32、MIG2、SIP2、SPT16和CHC1）、POX1的5'部分以及另外两个完整的、超过100个氨基酸的未鉴定开放阅读框。

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