Clough R, Statnick M, Maring-Smith M, Wang C, Eells J, Browning R, Dailey J, Jobe P
Department of Anatomy, Southern Illinois University School of Medicine-Carbondale, IL, USA.
Neuroreport. 1996 Dec 20;8(1):341-6. doi: 10.1097/00001756-199612200-00067.
This study investigated whether transplantation of fetal raphe tissue into genetically epilepsy-prone rats (GEPR-3s) would reduce the severity of seizures previously exacerbated by depletion of brain serotonin. Mild-seizure GEPR-3s were depleted of brain serotonin by 5,7-dihydroxytryptamine (DHT) and evaluated for seizure severity. Rats then received 15-day fetal raphe tissue, fetal neocortical tissue or were sham grafted. GEPR-3s treated with 5,7-DHT showed increased seizure severity following depletion of serotonin and subsequent reductions in severity as a result of fetal raphe transplantation. Sham- or neocortex-grafted rats maintained elevated seizure severity scores throughout the study. Prominent raphe or cortical grafts were observed within the third ventricle of GEPRs at autopsy. These findings show that transplantation of fetal raphe tissue promotes lasting reductions in increased seizure severity resulting from depletion of serotonin in the GEPR brain.
本研究调查了将胎鼠中缝组织移植到遗传性癫痫易感大鼠（GEPR-3s）体内，是否会减轻先前因脑血清素耗竭而加剧的癫痫发作严重程度。通过5,7-二羟基色胺（DHT）使轻度癫痫发作的GEPR-3s脑内血清素耗竭，并评估癫痫发作严重程度。然后，大鼠接受15天龄的胎鼠中缝组织、胎鼠新皮质组织移植，或进行假手术移植。用5,7-DHT处理的GEPR-3s在血清素耗竭后癫痫发作严重程度增加，随后由于胎鼠中缝组织移植而使严重程度降低。在整个研究过程中，接受假手术或新皮质移植的大鼠癫痫发作严重程度评分一直居高不下。尸检时在GEPRs的第三脑室内观察到明显的中缝或皮质移植物。这些发现表明，胎鼠中缝组织移植可使GEPR脑内血清素耗竭导致的癫痫发作严重程度增加得到持久减轻。
