Restriction site mapping of a bovine adenovirus type 10 strain.

Physical maps of the genome of a bovine adenovirus (BAV) type 10 strain (isolate Belfast1) were constructed for eight restriction enzymes. The size of the viral genome was estimated to be around 29,800 base pairs (bp). The orientation of the maps was determined on the basis of partial DNA sequences of the cloned PstI/D fragment of Belfast1. This work is an introduction to DNA sequence and phylogenetic analysis of BAV-10 in order to clarify its taxonomic place.