Benkö M, Bartha A, Wadell G
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest.
Intervirology. 1988;29(6):346-50. doi: 10.1159/000150066.
Eight reference strains of the nine accepted bovine adenovirus serotypes were compared by means of restriction enzyme (RE) analysis of the viral DNA. The study was carried out with the aim to identify RE giving restriction patterns which are particularly feasible for identification of new isolates and useful for their classification into subgroups. The BamHI, EcoRI, HindIII and PstI enzymes were feasible for identification of strains. The classification of bovine adenovirus (BAV) into subgroup 1 (BAV-1, 2, 3 and 9) and subgroup 2 (BAV-4, 5, 6, 7 and 8) was supported by (i) the significant difference in genome size between members of subgroup 1 (ranging from 32.73 to 37.67 kb) and members of subgroup 2 (ranging from 28.78 to 31.18 kb); and (ii) the observation that the nucleotide sequence recognized by EcoRI is less frequent in the genomes of subgroup 1 members - yielding 4-7 RE fragments - than in the genomes of subgroup 2 members - yielding 10-16 RE fragments.
通过对病毒DNA进行限制性内切酶（RE）分析，对9种公认的牛腺病毒血清型中的8种参考菌株进行了比较。开展这项研究的目的是确定能产生特别适合鉴定新分离株且有助于将其分类到亚组的限制性图谱的限制性内切酶。BamHI、EcoRI、HindIII和PstI酶对于菌株鉴定是可行的。牛腺病毒（BAV）分为第1亚组（BAV - 1、2、3和9）和第2亚组（BAV - 4、5、6、7和8），这得到了以下两方面的支持：（i）第1亚组成员（基因组大小范围为32.73至37.67 kb）和第2亚组成员（基因组大小范围为28.78至31.18 kb）之间基因组大小存在显著差异；（ii）观察到EcoRI识别的核苷酸序列在第1亚组成员基因组中出现的频率较低，产生4 - 7个RE片段，而在第2亚组成员基因组中出现的频率较高，产生10 - 16个RE片段。
