从一株对头孢他啶耐药但对头孢噻吩敏感的大肠杆菌分离株中获得的新型超广谱TEM型β-内酰胺酶
Novel extended-spectrum TEM-type beta-lactamase from an Escherichia coli isolate resistant to ceftazidime and susceptible to cephalothin.
作者信息
Chanal-Claris C, Sirot D, Bret L, Chatron P, Labia R, Sirot J
机构信息
Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Clermont-Ferrand, France.
出版信息
Antimicrob Agents Chemother. 1997 Mar;41(3):715-6. doi: 10.1128/AAC.41.3.715.
Abstract
A novel extended-spectrum TEM-type beta-lactamase was detected in an Escherichia coli isolate which was resistant to ceftazidime and susceptible to cephalothin. The corresponding bla gene was sequenced. The deduced amino acid sequence showed the following three amino acid replacements with respect to the TEM-2 sequence: Glu-->Lys-104, Arg-->Ser-164, and Glu-->Lys-240. Since it confers a ceftazidimase-type resistance phenotype, we propose for this novel enzyme the designation CAZ-9, corresponding to TEM-46 in the sequential numbering scheme of TEM beta-lactamases.
摘要
在一株对头孢他啶耐药但对头孢噻吩敏感的大肠杆菌分离株中检测到一种新型的超广谱TEM型β-内酰胺酶。对相应的bla基因进行了测序。推导的氨基酸序列与TEM-2序列相比有以下三个氨基酸替换：第104位谷氨酸（Glu）替换为赖氨酸（Lys），第164位精氨酸（Arg）替换为丝氨酸（Ser），第240位谷氨酸（Glu）替换为赖氨酸（Lys）。由于它赋予了头孢他啶酶型耐药表型，我们建议将这种新型酶命名为CAZ-9，在TEMβ-内酰胺酶的连续编号方案中对应于TEM-46。
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