Nakanishi H, Sun Y, Nakamura R K, Mori K, Ito M, Suda S, Namba H, Storch F I, Dang T P, Mendelson W, Mishkin M, Kennedy C, Gillin J C, Smith C B, Sokoloff L
Laboratory of Cerebral Metabolism, National Institute of Mental Health, Bethesda, MD 20892-4030, USA.
Eur J Neurosci. 1997 Feb;9(2):271-9. doi: 10.1111/j.1460-9568.1997.tb01397.x.
Local rates of cerebral protein synthesis (ICPSleu) were determined with the autoradiographic L-[1-14C]leucine method in seven awake and seven asleep, adult rhesus monkeys conditioned to sleep in a restraining chair in a darkened, ventilated chamber while EEG, EOG, and EMG were monitored. Prior to the period of measurement all animals slept for 1-4 h. Controls were awakened after at least one period of rapid-eye-movement (REM) sleep. Experimental animals were allowed to remain asleep, and they exhibited non-REM sleep for 71-99% of the experimental period. Statistically significant differences in ICPSleu between control and experimental animals were found in four of the 57 regions of brain examined, but these effects may have occurred by chance. In the sleeping animals, however, correlations between ICPSleu and percent time in deep sleep were positive in all regions and were statistically significant (P < or = 0.05) in 35 of the regions. When time in deep sleep was weighted for the integrated specific activity of leucine in grey matter, positive correlations were statistically significant (P < or = 0.05) in 18 regions in the experimental animals. These results suggest that rates of protein synthesis are increased in many regions of the brain during deep sleep compared with light sleep.
采用放射自显影L-[1-14C]亮氨酸法，对7只清醒和7只睡眠状态的成年恒河猴进行脑蛋白合成局部速率（ICPSleu）的测定。这些猴子被训练在黑暗、通风的实验室内的约束椅上睡眠，同时监测脑电图（EEG）、眼电图（EOG）和肌电图（EMG）。在测量前，所有动物均先睡眠1 - 4小时。对照组在至少经历一个快速眼动（REM）睡眠期后被唤醒。实验动物则继续保持睡眠状态，在实验期间非快速眼动睡眠占71 - 99%。在所检查的57个脑区中，有4个脑区发现对照组和实验动物之间的ICPSleu存在统计学显著差异，但这些差异可能是偶然出现的。然而，在睡眠动物中，所有脑区的ICPSleu与深睡眠所占时间百分比之间均呈正相关，且在35个脑区中具有统计学显著性（P≤0.05）。当根据灰质中亮氨酸的综合比活性对深睡眠时间进行加权时，实验动物中有18个脑区的正相关具有统计学显著性（P≤0.05）。这些结果表明，与浅睡眠相比，深睡眠期间大脑许多区域的蛋白合成速率增加。
