Feng J, Liu R, Wu G, Tang S
Department of Physiology, Faculty of Medicine, Research Centre, Hôpital du Sacré-Coeur de Montréal, Université de Montréal, Québec, Canada.
Mol Cell Biochem. 1997 Feb;167(1-2):153-8. doi: 10.1023/a:1006837606488.
We examined the effect on 6-Keto-PGF1a and TXB2 (the stable metabolites of PGI2 and TXA2) outflow of pretreatment with tetramethylpyrazine in the hypoxic isolated rat heart. In control hearts, 6-Keto-PGF1a was increased from 185 +/- 46 pg/ml of baseline value to 335 +/- 76 pg/ml after 2 min of hypoxia and TXB2 increased from 136 +/- 28 pg/ml of baseline value to 230 +/- 43 pg/ml at 20 min of hypoxia and 252 +/- 32 pg/ml after 5 min of reoxygenation. Pretreatment with tetramethylpyrazine increased the 6-Keto-PGF1a concentration to 266 +/- 51 pg/ml (143% of control heart), 471 +/- 89 pg/ml (150% of control heart) and 332 +/- 47 pg/ml (195% of control heart) at 15 min of normoxia, 2 min of hypoxia and 5 min of reoxygenation, respectively (p < 0.05 vs control). On the other hand, tetramethylpyrazine diminished the release of TXB2 to 78 +/- 21 pg/ml (174% of control heart), 160 +/- 30 pg/ml (144% of control heart), and 196 +/- 23 pg/ml (128% of control heart) at 15 min of normoxia, 20 min of hypoxia and 5 min of reoxygenation, respectively (p < 0.05 vs control). These data show that pretreatment with tetramethylpyrazine enhances PGI2 outflow and attenuates release of TXA2 in the rat heart during normoxia, hypoxia and reoxygenation.
我们研究了川芎嗪预处理对缺氧离体大鼠心脏中6-酮-前列腺素F1α（6-Keto-PGF1α）和血栓素B2（TXB2，前列环素（PGI2）和血栓素A2（TXA2）的稳定代谢产物）流出的影响。在对照心脏中，缺氧2分钟后，6-酮-前列腺素F1α从基线值185±46 pg/ml增加到335±76 pg/ml，缺氧20分钟时TXB2从基线值136±28 pg/ml增加到230±43 pg/ml，复氧5分钟后增加到252±32 pg/ml。川芎嗪预处理使常氧15分钟、缺氧2分钟和复氧5分钟时6-酮-前列腺素F1α浓度分别增加到266±51 pg/ml（对照心脏的143%）、471±89 pg/ml（对照心脏的150%）和332±47 pg/ml（对照心脏的195%）（与对照相比，p<0.05）。另一方面，川芎嗪使常氧15分钟、缺氧20分钟和复氧5分钟时TXB2的释放分别减少到78±21 pg/ml（对照心脏的174%）、160±30 pg/ml（对照心脏的144%）和196±23 pg/ml（对照心脏的128%）（与对照相比，p<0.05）。这些数据表明，川芎嗪预处理可增强大鼠心脏在常氧、缺氧和复氧期间前列环素的流出并减弱血栓素A2的释放。
