Wang X, Zhu S, Liu Q, Hu A, Zan Z, Yu Q, Yin Q
Department of Parasitology, West China University of Medical Sciences, Chengdu, Sichuan, China.
Bull World Health Organ. 1996;74(6):599-603.
The QBC (quantitative buffy coat) technique was compared with that of the Giemsa-stained thick blood film (GTF) under field conditions in Junlian and Mingshan counties, Sichuan, China, for rapid diagnosis of vivax malaria. Blood samples were collected from 364 volunteer villagers, and each sample was examined with both the QBC and GTF techniques. For each GTF sample (10 microliters of blood), as many as 300 oil-immersion fields were examined; each QBC tube was inspected for up to 5 minutes. The GTF technique resulted in 86 positive blood samples and 278 negative; the QBC technique indicated 89 positive and 275 negative samples. Relative to the results obtained with GTF, the QBC technique had a sensitivity and specificity of 87.2% and 95.0%, respectively; concordance between the tests was 93.1%. The median time-to-positive diagnosis with the QBC technique (1.12 min) was 11% of that with GTF. The distribution of different developmental stages of Plasmodium vivax parasites was also examined in the centrifuged QBC tubes: all stages except schizonts could be found in the lower part of the platelet zone (the interphase between the monocyte and platelet layers), especially ring forms.
在中国四川筠连县和名山县的现场条件下，对定量血沉棕黄层（QBC）技术与吉姆萨染色厚血膜（GTF）技术进行了比较，以快速诊断间日疟。从364名志愿者村民中采集血样，每个样本分别采用QBC和GTF技术进行检测。对于每个GTF样本（10微升血液），检查多达300个油镜视野；每个QBC管检查长达5分钟。GTF技术检测出86份阳性血样和278份阴性血样；QBC技术检测出89份阳性和275份阴性样本。相对于GTF的检测结果，QBC技术的灵敏度和特异度分别为87.2%和95.0%；两种检测方法的一致性为93.1%。QBC技术阳性诊断的中位时间（1.12分钟）为GTF的11%。还在离心的QBC管中检查了间日疟原虫不同发育阶段的分布：除裂殖体阶段外，所有阶段均可在血小板区下部（单核细胞层和血小板层之间的中间相）发现，尤其是环状体。
