Liu Q, Zhu S, Mizuno S, Kimura M, Liu P, Isomura S, Wang X, Kawamoto F
Department of International Health, Nagoya University School of Medicine, Nagoya 466-8550, Japan.
J Clin Microbiol. 1998 Nov;36(11):3378-81. doi: 10.1128/JCM.36.11.3378-3381.1998.
By two PCR-based diagnostic methods, Plasmodium malariae infections have been rediscovered at two foci in the Sichuan province of China, a region where no cases of P. malariae have been officially reported for the last 2 decades. In addition, a variant form of P. malariae which has a deletion of 19 bp and seven substitutions of base pairs in the target sequence of the small-subunit (SSU) rRNA gene was detected with high frequency. Alignment analysis of Plasmodium sp. SSU rRNA gene sequences revealed that the 5' region of the variant sequence is identical to that of P. vivax or P. knowlesi and its 3' region is identical to that of P. malariae. The same sequence variations were also found in P. malariae isolates collected along the Thai-Myanmar border, suggesting a wide distribution of this variant form from southern China to Southeast Asia.
通过两种基于聚合酶链反应（PCR）的诊断方法，在中国四川省的两个疫点重新发现了间日疟原虫感染，在过去20年里该地区未正式报告过间日疟原虫病例。此外，还高频检测到一种间日疟原虫的变异形式，其小亚基（SSU）核糖体RNA基因靶序列中有19个碱基对的缺失和7个碱基对的替换。疟原虫属SSU核糖体RNA基因序列的比对分析表明，变异序列的5'区域与间日疟原虫或诺氏疟原虫的5'区域相同，其3'区域与间日疟原虫的3'区域相同。在泰国-缅甸边境收集的间日疟原虫分离株中也发现了相同的序列变异，这表明这种变异形式在中国南部到东南亚广泛分布。
