流感嗜血杆菌编码的一种ATP依赖型DNA连接酶的特性分析。
Characterization of an ATP-dependent DNA ligase encoded by Haemophilus influenzae.
作者信息
Cheng C, Shuman S
机构信息
Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10021 USA.
出版信息
Nucleic Acids Res. 1997 Apr 1;25(7):1369-74. doi: 10.1093/nar/25.7.1369.
Abstract
We report that Haemophilus influenzae encodes a 268 amino acid ATP-dependent DNA ligase. The specificity of Haemophilus DNA ligase was investigated using recombinant protein produced in Escherichia coli. The enzyme catalyzed efficient strand joining on a singly nicked DNA in the presence of magnesium and ATP (Km = 0.2 microM). Other nucleoside triphosphates or deoxynucleoside triphosphates could not substitute for ATP. Haemophilus ligase reacted with ATP in the absence of DNA substrate to form a covalent ligase-adenylate intermediate. This nucleotidyl transferase reaction required a divalent cation and was specific for ATP. The Haemophilus enzyme is the first example of an ATP-dependent DNA ligase encoded by a eubacterial genome. It is also the smallest member of the covalent nucleotidyl transferase superfamily, which includes the bacteriophage and eukaryotic ATP-dependent polynucleotide ligases and the GTP-dependent RNA capping enzymes.
摘要
我们报道了流感嗜血杆菌编码一种含268个氨基酸的ATP依赖性DNA连接酶。利用在大肠杆菌中产生的重组蛋白对流感嗜血杆菌DNA连接酶的特异性进行了研究。该酶在镁离子和ATP存在的情况下，能高效催化单切口DNA上的链连接反应（Km = 0.2微摩尔）。其他核苷三磷酸或脱氧核苷三磷酸不能替代ATP。流感嗜血杆菌连接酶在没有DNA底物的情况下与ATP反应，形成共价连接酶 - 腺苷酸中间体。这种核苷酸转移酶反应需要二价阳离子，且对ATP具有特异性。流感嗜血杆菌酶是真细菌基因组编码的ATP依赖性DNA连接酶的首个实例。它也是共价核苷酸转移酶超家族中最小的成员，该超家族包括噬菌体和真核生物的ATP依赖性多核苷酸连接酶以及GTP依赖性RNA加帽酶。
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2
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Curr Biol. 1996 Mar 1;6(3):279-91. doi: 10.1016/s0960-9822(02)00478-5.
3
Expression and characterization of an RNA capping enzyme encoded by Chlorella virus PBCV-1.小球藻病毒PBCV-1编码的RNA封端酶的表达与特性分析
J Virol. 1996 Oct;70(10):6658-64. doi: 10.1128/JVI.70.10.6658-6664.1996.
4
Crystal structure of an ATP-dependent DNA ligase from bacteriophage T7.来自噬菌体T7的一种ATP依赖的DNA连接酶的晶体结构。
Cell. 1996 May 17;85(4):607-15. doi: 10.1016/s0092-8674(00)81260-x.
5
RNA capping enzyme and DNA ligase: a superfamily of covalent nucleotidyl transferases.RNA 封端酶与 DNA 连接酶：共价核苷酸转移酶超家族
Mol Microbiol. 1995 Aug;17(3):405-10. doi: 10.1111/j.1365-2958.1995.mmi_17030405.x.
6
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J Bacteriol. 1996 Jan;178(2):396-402. doi: 10.1128/jb.178.2.396-402.1996.
7
Vaccinia virus DNA ligase: specificity, fidelity, and inhibition.痘苗病毒DNA连接酶：特异性、保真度及抑制作用
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9
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10
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