Sabokbar A, Fujikawa Y, Brett J, Murray D W, Athanasou N A
Nuffield Department of Orthopaedic Surgery, University of Oxford, Nuffield Orthopaedic Centre, Headington, UK.
Acta Orthop Scand. 1996 Dec;67(6):593-8. doi: 10.3109/17453679608997763.
To determine the influence of polymethylmethacrylate (PMMA) wear particles on macrophage-osteoclast differentiation, PMMA particles were added to mouse monocytes which were cocultured with UMR 106 osteoblast-like cells in the presence of 1,25 dihydroxy vitamin D3[1,25(OH)2D3] for up to 7 days on glass coverslips and for up to 14 days on human cortical bone slices. An increase in osteoclast differentiation, as evidenced by the expression of the osteoclast-associated enzyme tartrate-resistant acid phosphatase (TRAP) and the extent of lacunar bone resorption, was observed in monocyte cultures to which PMMA had been added. Interleukin 4 (IL-4) and Leukemia Inhibitory Factor (LIF) added to these cocultures caused considerably less expression of TRAP and significant inhibition of lacunar bone resorption. This inhibitory effect was reversed by the addition of specific neutralizing antibodies to LIF and IL-4. These findings show that PMMA-wear particle-associated macrophages exhibit an enhanced capacity for differentiation to osteoclastic bone-resorbing cells.
为了确定聚甲基丙烯酸甲酯（PMMA）磨损颗粒对巨噬细胞向破骨细胞分化的影响，将PMMA颗粒添加到小鼠单核细胞中，这些单核细胞在1,25-二羟基维生素D3[1,25(OH)2D3]存在的情况下，与UMR 106成骨样细胞共培养，在玻璃盖玻片上培养长达7天，在人皮质骨切片上培养长达14天。在添加了PMMA的单核细胞培养物中观察到破骨细胞分化增加，这通过破骨细胞相关酶抗酒石酸酸性磷酸酶（TRAP）的表达和腔隙性骨吸收的程度得以证明。添加到这些共培养物中的白细胞介素4（IL-4）和白血病抑制因子（LIF）导致TRAP的表达明显减少，并显著抑制腔隙性骨吸收。通过添加针对LIF和IL-4的特异性中和抗体，这种抑制作用被逆转。这些发现表明，与PMMA磨损颗粒相关的巨噬细胞向破骨细胞样骨吸收细胞分化的能力增强。
