Jahnke V, Strange R, Matthias C, Fryer A
Department of Otorhinolaryngology, Virchow-Klinikum, Humboldt University, Berlin, Germany.
Eur Arch Otorhinolaryngol. 1997;254 Suppl 1:S147-9. doi: 10.1007/BF02439747.
While cigarette smoking and alcohol consumption have been linked to laryngeal squamous cell carcinoma (SCC), the role of genetic factors in determining individual susceptibility is unknown. We describe the role of allelism at the glutathione S-transferase GSTM1, GSTM3, GSTT1 and cytochrome P450 CYP1A1, CYP2E1, CYP2D6 loci in determining individual susceptibility to laryngeal SCC. Enzyme genotypes were determined using polymerase chain reaction and restriction enzyme digestion of leukocyte DNA collected from 269 patients with T1-T4 laryngeal carcinomas and 216 controls. While the frequencies of the heterozygote GSTM1 A/B genotype and the homozygote GSTM3 B/B genotype were statistically significantly lower in the patients with tumors than in controls, the frequency of the GSTT1 null genotype was higher in the patients than in controls. The data suggest that allelism at GST loci mediates susceptibility to SCC of the larynx. GSTM1 A/B and GSTM3 B/B appear to be associated with reduced risk, while GSTT1 null may confer increased risk. These findings are compatible with the view that genetic predisposition is important in determining risk for this cancer.
虽然吸烟和饮酒与喉鳞状细胞癌（SCC）有关，但遗传因素在决定个体易感性方面的作用尚不清楚。我们描述了谷胱甘肽S-转移酶GSTM1、GSTM3、GSTT1以及细胞色素P450 CYP1A1、CYP2E1、CYP2D6基因座的等位基因在决定个体对喉SCC易感性中的作用。使用聚合酶链反应和限制性内切酶消化从269例T1-T4期喉癌患者和216例对照者收集的白细胞DNA来确定酶基因型。虽然杂合子GSTM1 A/B基因型和纯合子GSTM3 B/B基因型的频率在肿瘤患者中统计学上显著低于对照组，但GSTT1无效基因型的频率在患者中高于对照组。数据表明，GST基因座的等位基因介导了对喉SCC的易感性。GSTM1 A/B和GSTM3 B/B似乎与风险降低有关，而GSTT1无效可能会增加风险。这些发现与遗传易感性在决定这种癌症风险中很重要的观点一致。
