Payne L N, Gillespie A M, Howes K
AFRC Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire, England.
Avian Dis. 1993 Apr-Jun;37(2):438-50.
Viruses rapidly able to transform cultured chicken bone marrow cells have been isolated from cases of myelocytic myeloid leukosis (MML) induced experimentally by the HPRS-103 strain of avian leukosis virus, and from field cases of MML. HPRS-103 virus itself did not acutely transform cultured bone-marrow cells. These findings suggest that during myeloid leukemogenesis by HPRS-103 virus, recombinant viruses are generated with transduced cellular oncogenes. The transformed cell appeared to be a macrophage precursor cell. Transformed cells in culture lost their proliferative capacity after a few weeks and then tended to resemble more differentiated macrophages. This change could be reversed temporarily by addition of a myelomonocytic growth factor, cMGF, to the culture medium. In oncogenicity tests, a selection of the virus strains induced MML, nephroblastomas, renal adenomas/adenocarcinomas, and other tumors in line 21 meat-type chickens but not in line 0 chickens. This difference may have been related to a propensity for the virus strains to induce persistent tolerant viremic infections in the line 21 chickens following infection at 1 day of age. The oncogenic pattern was not clearly related to the ability of the viruses to transform cultured bone-marrow cells. The generation of acutely transforming viruses during myeloid leukemogenesis may be relevant to the occurrence of MML in the field.
已从禽白血病病毒HPRS - 103株实验诱导的髓细胞性髓样白血病（MML）病例以及MML的田间病例中分离出能够迅速转化培养的鸡骨髓细胞的病毒。HPRS - 103病毒本身不会急性转化培养的骨髓细胞。这些发现表明，在HPRS - 103病毒引起的髓样白血病发生过程中，会产生带有转导细胞癌基因的重组病毒。转化细胞似乎是巨噬细胞前体细胞。培养中的转化细胞在几周后失去增殖能力，然后倾向于更类似于分化的巨噬细胞。通过向培养基中添加髓单核细胞生长因子cMGF，这种变化可以暂时逆转。在致癌性试验中，选择的病毒株在21系肉用型鸡中诱导了MML、肾母细胞瘤、肾腺瘤/腺癌和其他肿瘤，但在0系鸡中未诱导。这种差异可能与病毒株在1日龄感染后在21系鸡中诱导持续耐受病毒血症感染的倾向有关。致癌模式与病毒转化培养骨髓细胞的能力没有明显关系。髓样白血病发生过程中急性转化病毒的产生可能与田间MML的发生有关。
