Christensen M M, Høy C E
Department of Biochemistry and Nutrition, Technical University of Denmark, Lyngby, Denmark.
Lipids. 1997 Feb;32(2):185-91. doi: 10.1007/s11745-997-0023-2.
Newborn rats were fed liquid diets containing 7 wt% fat in which 3.8% of the total fatty acids were 22:6n-3. The fats were either a specific structured oil with 22:6n-3 mostly located in the sn-2 position or a randomized oil with 22:6n-3 equally distributed in the triacylglycerol (TAG) molecules. The oils were manufactured by interesterification of fish oil TAG with free fatty acids from butterfat. The pups were tube-fed three times a day and stayed with their dams during the night. After 14 d they were fed solid diets containing the same oils for the next 7 d. A reference group stayed with the dams and received ordinary rat chow at weaning. In general no significant differences between the two dietary treatments were observed in the tissues examined except for adipose tissue. The levels of 22:6n-3 were significantly increased in brain phosphatidylcholines (PC) and phosphatidylserines (PS) of both experimental groups compared with the reference group after three weeks, whereas no differences were found in brain phosphatidylethanolamines (PE) and phosphatidylinositols (PI). In all groups and all phospholipids examined, the levels of 20:4n-6 generally decreased from 1 to 3 wk and were significantly lower in the experimental groups compared with the reference group at 3 wk except for PI. In liver, PC and PE 22:6n-3 remained constant in the experimental groups but decreased significantly in the reference group, whereas in liver PS 22:6n-3 increased in all groups, but reached significantly higher levels in the experimental groups than in the reference group. In adipose tissue, 22:6n-3 increased in the experimental groups during the study period, but decreased in the reference group, suggesting that a surplus of dietary 22:6n-3 was stored.
新生大鼠喂食含7 wt%脂肪的液体饮食，其中3.8%的总脂肪酸为22:6n-3。这些脂肪要么是一种特定的结构化油，22:6n-3主要位于sn-2位置，要么是一种随机化油，22:6n-3均匀分布在三酰甘油（TAG）分子中。这些油是通过鱼油TAG与来自乳脂肪的游离脂肪酸进行酯交换反应制造的。幼崽每天通过管饲喂食三次，夜间与母鼠待在一起。14天后，它们在接下来的7天里喂食含有相同油类的固体饮食。一个对照组与母鼠待在一起，并在断奶时接受普通大鼠饲料。总体而言，除脂肪组织外，在所检查的组织中，两种饮食处理之间未观察到显著差异。与对照组相比，三周后两个实验组脑磷脂酰胆碱（PC）和磷脂酰丝氨酸（PS）中的22:6n-3水平显著升高，而脑磷脂酰乙醇胺（PE）和磷脂酰肌醇（PI）中未发现差异。在所有组和所检查的所有磷脂中，20:4n-6水平通常从第1周降至第3周，除PI外，在第3周时实验组显著低于对照组。在肝脏中，实验组的PC和PE 22:6n-3保持恒定，但对照组显著下降，而肝脏PS 22:6n-3在所有组中均升高，但实验组显著高于对照组。在脂肪组织中，实验组在研究期间22:6n-3增加，而对照组下降，这表明饮食中多余的22:6n-3被储存起来。
