Sharma Alok K, Schultze Albert E, Cooper Dale M, Reams Rachel Y, Jordan William H, Snyder Paul W
Department of Pathology, Lilly Research Laboratories, Division of Eli Lilly and Co., Greenfield, Indiana, 46140, USA.
Toxicol Pathol. 2006;34(4):393-5. doi: 10.1080/01926230600798609.
A simple technique for cerebrospinal fluid (CSF) collection was developed in F-344 rats. Cell counts and total protein concentrations were evaluated to assess sample quality. While the 50 to 70 mu L samples of CSF collected on three different days showed a progressive decrease in the total erythrocyte and nucleated cell counts, no significant changes were observed in the total protein concentrations. Progressive decreases in the total erythrocyte count correlated positively with the decreases in volume of CSF collected. Our data suggest that collection of less than 50 mu L of CSF will give a better quality of CSF in F-344 rats. This is the first report of cellular and protein parameters in the CSF of F-344 rats.
在F-344大鼠中开发了一种简单的脑脊液（CSF）采集技术。对细胞计数和总蛋白浓度进行评估以评估样本质量。虽然在三个不同日期采集的50至70微升脑脊液样本显示总红细胞和有核细胞计数逐渐减少，但总蛋白浓度未观察到显著变化。总红细胞计数的逐渐减少与采集的脑脊液体积减少呈正相关。我们的数据表明，采集少于50微升的脑脊液将使F-344大鼠的脑脊液质量更好。这是关于F-344大鼠脑脊液中细胞和蛋白质参数的首次报告。
