Roberts SC, Shuler ML
School of Chemical Engineering 120 Olin Hall Cornell University Ithaca, NY 14853-5201, USA
Curr Opin Biotechnol. 1997 Apr 1;8(2):154-9. doi: 10.1016/s0958-1669(97)80094-8.
Progress towards the commercial-scale use of plant cell cultures over the past three years has been significant. Elicitation, particularly with methyl jasmonate, has been effective at increasing the product yields of a wide variety of secondary metabolites, particularly when it is applied synergistically with enhancement strategies such as immobilization and in situ extraction. Rapid advances in understanding the regulation of the biosynthetic pathways of secondary metabolites are allowing the application of enhancement strategies to move from empirical to semirational. Much of this progress is exemplified by work on paclitaxel (Taxol), where yields have improved more than 100-fold in the past two years.
在过去三年里，植物细胞培养商业化规模应用方面取得了重大进展。诱导，尤其是使用茉莉酸甲酯进行诱导，已有效地提高了多种次生代谢产物的产量，特别是当它与固定化和原位提取等强化策略协同应用时。在理解次生代谢产物生物合成途径调控方面的迅速进展，使得强化策略的应用从经验性走向半理性。这一进展在紫杉醇（泰素）的研究工作中得到了充分体现，在过去两年里其产量提高了100多倍。
