Institute of Fungus Resources, Guizhou University, Guiyang, China.
Folia Microbiol (Praha). 2013 Jul;58(4):283-9. doi: 10.1007/s12223-012-0203-9. Epub 2012 Nov 16.
Some endophyte isolates were isolated in a bamboo pole sample parasitized the fungus Shiraia bambusicola from Zhejiang Province. After screening through hypocrellin bacteriostatic effect and fermentation test, we got the isolate TX4 of bacterial elicitor and GZUIFR-TT1 of fungal elicitor which had certain effect to promote S. bambusicola to produce hypocrellin. The Plackett-Burman design was introduced to evaluate the effects of nine factors based on single-factor test. Yeast extract, glucose, and isolate GZUIFR-TT1 elicitor were found to be the critical activity factors for increasing the total hypocrellin production. So we identified the isolate GZUIFR-TT1 as Trametes sp. Through response surface methodology, we obtained the optimum production conditions as follows: yeast extract, 2.99 g/L; glucose, 32.45 g/L; and Trametes sp. elicitor, 81.40 μg/mL. Under the above conditions, the experimental value of hypocrellin production was 102.60 mg/L, compared with the control it increased about 7.90 times.
一些内生菌分离株从浙江省受真菌感染的寄生竹杆样本中分离出来。经过对竹红菌素抑菌作用和发酵试验的筛选，我们得到了细菌诱导子 TX4 和真菌诱导子 GZUIFR-TT1，它们对促进 Shiraia bambusicola 产生竹红菌素有一定的作用。基于单因素试验，我们采用 Plackett-Burman 设计来评估九个因素的影响。酵母提取物、葡萄糖和 GZUIFR-TT1 诱导子被发现是提高总竹红菌素产量的关键活性因素。因此，我们确定该分离株 GZUIFR-TT1 为 Trametes sp. 通过响应面法，我们得到了最佳的生产条件如下：酵母提取物 2.99 g/L；葡萄糖 32.45 g/L；和 Trametes sp. 诱导子 81.40 μg/mL。在上述条件下，竹红菌素产量的实验值为 102.60 mg/L，与对照相比增加了约 7.90 倍。
