Saxton M J
Institute of Theoretical Dynamics, University of California, Davis 95616, USA.
Biophys J. 1997 Apr;72(4):1744-53. doi: 10.1016/S0006-3495(97)78820-9.
In single-particle tracking experiments, the diffusion coefficient D may be measured from the trajectory of an individual particle in the cell membrane. The statistical distribution of single-trajectory diffusion coefficients is examined by Monte Carlo calculations. The width of this distribution may be useful as a measure of the heterogeneity of the membrane and as a test of models of hindered diffusion in the membrane. For some models, the distribution of the short-range diffusion coefficient is much narrower than the observed distribution for proteins diffusing in cell membranes. To aid in the analysis of single-particle tracking measurements, the distribution of D is examined for various definitions of D and for various trajectory lengths.
在单粒子追踪实验中，扩散系数D可通过细胞膜中单个粒子的轨迹来测量。单轨迹扩散系数的统计分布通过蒙特卡罗计算来检验。该分布的宽度可作为衡量膜异质性的指标以及膜中受阻扩散模型的检验。对于某些模型，短程扩散系数的分布比在细胞膜中扩散的蛋白质的观测分布窄得多。为了辅助单粒子追踪测量的分析，针对D的各种定义以及各种轨迹长度对D的分布进行了检验。
