Schulze K, Christensen J
Gastroenterology. 1977 Nov;73(5):1082-5.
To seek a possible role of estrogen and progesterone in the development of changes in esophageal function during pregnancy, we produced a state of pseudopregnancy by administration of hormones to a suitable animal model. Twenty-two female opossums weighing an average of 2.5 kg, were divided into two groups. The treated group received intramuscular injections of 100 microgram of estradiol valerate daily from day 1 to day 12 and 15 mg of medroxyprogesterone acetate from day 7 to day 12. The control group received no injections. Both groups underwent manometry on days 1, 7, and 12. Lower esophageal sphincter pressure decreased (P less than 0.05) in treated animals from 58 +/- 13 mm Hg and 57 +/- 11 on days 1 and 7, respectively, to 44 +/- 10 mm Hg on day 12. The lower esophageal sphincter pressure remained unchanged in control animals. In both groups, there was no change in peristaltic wave pressure, duration, or velocity in the distal 6 cm of the esophagus. No abnormal peristaltic phenomena were observed. Esophageal muscle strips prepared from treated animals showed responses to electrical field stimulation of intrinsic nerves that were like those from control animals. The same was true for responses to acetylcholine and pentagastrin. Total tissue water and total tissue potassium content did not differ in treatment and control animals.
为探究雌激素和孕激素在孕期食管功能变化发展过程中可能发挥的作用，我们通过向合适的动物模型注射激素制造了假孕状态。22只平均体重为2.5千克的雌性负鼠被分为两组。治疗组从第1天至第12天每天接受100微克戊酸雌二醇的肌肉注射，从第7天至第12天接受15毫克醋酸甲羟孕酮的注射。对照组未接受注射。两组在第1天、第7天和第12天均进行了测压。治疗组动物的食管下括约肌压力从第1天的58±13毫米汞柱和第7天的57±11毫米汞柱分别降至第12天的44±10毫米汞柱（P<0.05）。对照组动物的食管下括约肌压力保持不变。两组中，食管远端6厘米处的蠕动波压力、持续时间或速度均无变化。未观察到异常蠕动现象。从治疗组动物制备的食管肌条对固有神经电场刺激的反应与对照组动物相似。对乙酰胆碱和五肽胃泌素的反应也是如此。治疗组和对照组动物的总组织含水量和总组织钾含量没有差异。
