白纹伊蚊的Hsp70热休克基因家族。

The Hsp70 heat-shock gene family of the mosquito Anopheles albimanus.

作者信息

Benedict M Q, Cockburn A F, Seawright J A

机构信息

USDA/ARS Medical and Veterinary Entomology Research Laboratory, Gainesville, Florida, USA.

出版信息

Insect Mol Biol. 1993;2(2):93-102. doi: 10.1111/j.1365-2583.1993.tb00130.x.

DOI:10.1111/j.1365-2583.1993.tb00130.x

PMID:9087548

Abstract

Four Hsp70 genes of the malaria vector Anopheles albimanus were isolated from a genomic DNA library as two non-overlapping clones each containing a pair of divergently transcribed genes having 75% DNA sequence similarity to the protein-coding regions of the Drosophila melanogaster Hsp70 genes. The clones were assigned to two loci on chromosome 2R by in situ hybridization. These clones hybridize strongly to heat-shock but only weakly to non-shocked mosquito RNA. The Hsp70 gene family of A. albimanus is undergoing concerted evolution probably by gene conversion. The general arrangement of the genes suggests that divergently transcribed pairs of genes at two loci is an ancient Dipteran arrangement predating the Nematocera/Cyclorrapha divergence.

摘要

从基因组DNA文库中分离出疟蚊白纹伊蚊的四个热休克蛋白70（Hsp70）基因，得到两个不重叠的克隆，每个克隆包含一对反向转录的基因，其与黑腹果蝇Hsp70基因的蛋白质编码区具有75%的DNA序列相似性。通过原位杂交将这些克隆定位到2R染色体上的两个位点。这些克隆与热休克后的蚊子RNA强烈杂交，但与未受刺激的蚊子RNA杂交较弱。白纹伊蚊的Hsp70基因家族可能通过基因转换正在经历协同进化。基因的总体排列表明，两个位点上反向转录的基因对是双翅目古老的排列方式，早于长角亚目/环裂亚目分歧。