Koffler D, Faiferman I, Gerber M A
Science. 1977 Nov 18;198(4318):741-3. doi: 10.1126/science.910157.
A radioimmunoassay for the detection of antibodies in human serum to tritium-labeled HeLa cell cytoplasmic ribosomes was developed with the use of Macaloid for the inhibition of endogenous ribonuclease activity. Antibodies were observed in the serum of patients with systemic lupus erythematosus in high incidence and titer. Patients with rheumatoid arthritis and chronic active hepatitis manifested a lower incidence and titer of antibodies to ribosomes, whereas serums from normal individuals and from patients with sarcoidosis, chronic glomerulonephritis, and malignant tumors showed no significant reactivity with cytoplasmic ribosomes. Maximum inhibition of the reaction was achieved with unlabeled HeLa cell ribosomes or rat liver ribosomes and partial inhibition by purified ribosomal RNA.
利用麦饭石抑制内源性核糖核酸酶活性，开发了一种用于检测人血清中针对氚标记的HeLa细胞胞质核糖体抗体的放射免疫测定法。在系统性红斑狼疮患者的血清中观察到抗体的高发生率和高滴度。类风湿性关节炎和慢性活动性肝炎患者的核糖体抗体发生率和滴度较低，而正常个体以及结节病、慢性肾小球肾炎和恶性肿瘤患者的血清与胞质核糖体无明显反应性。未标记的HeLa细胞核糖体或大鼠肝脏核糖体可实现反应的最大抑制，而纯化的核糖体RNA可实现部分抑制。
