Koffler D, Miller T E, Lahita R G
Arthritis Rheum. 1979 May;22(5):463-70. doi: 10.1002/art.1780220505.
The specificity of antibodies reactive with cytoplasmic ribosomes detected in systemic lupus erythematosus (SLE) sera was studied by a radioimmunoassay procedure with 3H labeled HeLa cell polysomes. The antibodies were directed primarily against RNA or a ribosomal RNA-protein complex (rRNP). Anti-RNA antibodies exhibited comparable reactivity with ribosomal RNA, nuclear RNA, structural RNA, and a slightly lower degree of reactivity with low molecular weight RNA. Anti-rRNP antibodies may be detected by precipitation in agar gel and appear to require both the RNA and protein moieties of ribosomes for reactivity. SLE sera containing only anti-rRNP antibodies are limited to patients with active disease. Sera from patients with both active and inactive SLE contain either anti-RNA or a mixture of anti-RNA and anti-rRNP antibodies. The possible participation of anti-ribosomal antibodies in immune complex formation is discussed.
采用以3H标记的HeLa细胞多核糖体的放射免疫分析方法，研究了系统性红斑狼疮（SLE）血清中与细胞质核糖体发生反应的抗体的特异性。这些抗体主要针对RNA或核糖体RNA - 蛋白质复合物（rRNP）。抗RNA抗体与核糖体RNA、核RNA、结构RNA表现出相当的反应性，而与低分子量RNA的反应性略低。抗rRNP抗体可通过琼脂凝胶沉淀检测到，并且似乎需要核糖体的RNA和蛋白质部分才能发生反应。仅含有抗rRNP抗体的SLE血清仅限于患有活动性疾病的患者。患有活动性和非活动性SLE的患者的血清含有抗RNA或抗RNA和抗rRNP抗体的混合物。本文讨论了抗核糖体抗体在免疫复合物形成中的可能作用。
