Wekesa J W, Yuval B, Washino R K
Department of Entomology, University of California, Davis 95616, USA.
J Med Entomol. 1997 Mar;34(2):219-25. doi: 10.1093/jmedent/34.2.219.
Multiple blood feeding in field populations of Anopheles freeborni Aitken and Culex tarsalis Coquillet was detected using a histologic technique. Examination of 333 blood engorged An. freeborni and 41 Cx. tarsalis females revealed that 13 and 10% of these females imbibed multiple blood meals, respectively. Spatial and temporal distribution of multiple blood feeding events was evaluated against the percentage of blood fed females and the abundance of female mosquitoes. The percentage of blood fed An. freeborni females was higher among those collected in pasture and riparian habitats than those collected in rice field and mixed habitats. Conversely, spatial variation of multiple blood feeding in An. freeborni was not significantly associated with rice field, pasture, riparian, or mixed habitats. The decrease of multiple blood feeding for An. freeborni through the season was correlated inversely with the increase of the abundance of adult females; but not with the percentage of blood fed females. These results confirmed that multiple blood meals in An. freeborni, and perhaps in Cx. tarsalis, are a frequent phenomenon, and that the histologic procedure is an appropriate tool for longitudinal and region-wide detection of multiple blood feeding necessary in epidemiologic studies.
采用组织学技术检测了弗氏按蚊和致倦库蚊野外种群中的多次吸血现象。对333只饱血的弗氏按蚊和41只致倦库蚊雌蚊进行检查后发现，这些雌蚊中分别有13%和10%吸食了多次血餐。根据饱血雌蚊的百分比和雌蚊数量评估了多次吸血事件的时空分布。在牧场和河岸栖息地采集的弗氏按蚊饱血雌蚊百分比高于在稻田和混合栖息地采集的。相反，弗氏按蚊多次吸血的空间变化与稻田、牧场、河岸或混合栖息地没有显著关联。随着季节推移，弗氏按蚊多次吸血现象的减少与成年雌蚊数量的增加呈负相关，但与饱血雌蚊的百分比无关。这些结果证实，弗氏按蚊以及可能还有致倦库蚊的多次吸血是一种常见现象，并且组织学方法是流行病学研究中纵向和区域范围检测多次吸血所需的合适工具。
