Tassios P T, Vatopoulos A C, Xanthaki A, Mainas E, Goering R V, Legakis N J
Department of Microbiology, Medical School, National University of Athens, Greece.
Eur J Clin Microbiol Infect Dis. 1997 Feb;16(2):170-3. doi: 10.1007/BF01709481.
Methicillin-resistant Staphylococcus aureus (MRSA) strains isolated over a one-year period from a Greek hospital were tested for their levels of resistance to methicillin by population analysis. Heterogeneously resistant strains belonged to classes I, II, and II/III, whereas homogeneously resistant ones belonged to class IV. Strains of all classes possessed the mecA gene. Pulsed-field gel electrophoresis (PFGE) of Smal-digested genomic DNA revealed that all heterogeneously resistant strains were also closely related, but in a cluster distinct from the heterogeneous one. The methicillin-sensitive strains displayed a greater variety of PFGE types compared to MRSA isolates.
通过群体分析，对从一家希腊医院在一年时间内分离出的耐甲氧西林金黄色葡萄球菌（MRSA）菌株进行了甲氧西林耐药水平检测。异质性耐药菌株属于I类、II类和II/III类，而均一性耐药菌株属于IV类。所有类别的菌株都拥有mecA基因。经Smal酶切的基因组DNA的脉冲场凝胶电泳（PFGE）显示，所有异质性耐药菌株也密切相关，但在一个与均一性耐药菌株不同的簇中。与MRSA分离株相比，甲氧西林敏感菌株显示出更多样化的PFGE类型。
