Schbath S
I.N.R.A., Unité de Biométrie, Jouy-en-Josas, France.
J Comput Biol. 1997 Spring;4(1):61-82. doi: 10.1089/cmb.1997.4.61.
The aim of this paper is to provide general results for predicting progress in a physical mapping project by anchoring random clones, when clones and anchors are not homogeneously distributed along the genome. A complete physical map of the DNA of an organism consists of overlapping clones spanning the entire genome. Several schemes can be used to construct such a map, depending on the way that clones overlap. We focus here on the approach consisting of assembling clones sharing a common random short sequence called an anchor. Some mathematical analyses providing statistical properties of anchored clones have been developed in the stationary case. Modeling the clone and anchor processes as nonhomogeneous Poisson processes provides such an analysis in a general nonstationary framework. We apply our results to two natural nonhomogeneous models to illustrate the effect of inhomogeneity. This study reveals that using homogeneous processes for clones and anchors provides an overly optimistic assessment of the progress of the mapping project.
本文的目的是在克隆和锚定物并非沿基因组均匀分布的情况下，通过锚定随机克隆来提供预测物理图谱绘制项目进展的一般结果。生物体DNA的完整物理图谱由跨越整个基因组的重叠克隆组成。根据克隆重叠的方式，可以使用几种方案来构建这样的图谱。我们在此关注的方法是组装共享一个称为锚定物的常见随机短序列的克隆。在平稳情况下，已经开展了一些提供锚定克隆统计特性的数学分析。将克隆和锚定过程建模为非齐次泊松过程可在一般的非平稳框架中进行此类分析。我们将结果应用于两个自然的非齐次模型，以说明非均匀性的影响。这项研究表明，对克隆和锚定物使用齐次过程会对图谱绘制项目的进展给出过于乐观的评估。
