Proksch E, Brasch J
Department of Dermatology, University of Kiel, Germany.
Acta Derm Venereol. 1997 Mar;77(2):102-4. doi: 10.2340/0001555577102104.
Previously, we have showed that artificial epidermal permeability barrier disruption leads to an increase in epidermal Langerhans' cell (LC) density within 24 h. We now asked if this is accompanied by an enhancement of allergic contact dermatitis. Barrier disruption was induced by acetone on the upper arms in 6 volunteers with known sensitization to nickel, fragrance mix, or p-phenylenediamine. Twenty-four hours after this treatment the relevant allergen was applied without occlusion or with Finn chambers. Twenty-four hours after application of the allergen, clinical grading and transepidermal water loss (TEWL) measurements were performed and biopsies were taken. Immunohistochemical stainings for LCs (anti-CD1a, Leu6) and for epidermal proliferation (Ki-S3) were performed. Open applications of the allergens after acetone pretreatment resulted in strong allergic test reactions. TEWL, which showed a 70% recovery 24 h after acetone treatment, was increased again 4-fold by the allergic test reactions. LC density, which was increased by 80% 24 h after acetone-induced barrier disruption, was further enhanced 2.4-fold in total. Epidermal proliferation showed a 6-fold increase after open application of the allergens. Under patch test conditions after acetone pretreatment very strong bullous reactions were observed. We conclude that the increase in epidermal LC density induced by epidermal permeability barrier disruption is accompanied by an enhanced response in allergic contact dermatitis.
此前，我们已经表明，人工破坏表皮通透性屏障会导致24小时内表皮朗格汉斯细胞（LC）密度增加。我们现在要问，这是否伴随着过敏性接触性皮炎反应增强。在6名已知对镍、香料混合物或对苯二胺过敏的志愿者上臂，用丙酮诱导屏障破坏。该处理24小时后，在不封闭或使用Finn Chambers的情况下涂抹相关变应原。涂抹变应原24小时后，进行临床分级和经表皮水分流失（TEWL）测量，并取活检组织。对LCs（抗CD1a、Leu6）和表皮增殖（Ki-S3）进行免疫组织化学染色。丙酮预处理后开放涂抹变应原导致强烈的过敏试验反应。TEWL在丙酮处理24小时后恢复70%，但过敏试验反应使其再次增加4倍。LC密度在丙酮诱导的屏障破坏24小时后增加80%，总体上进一步增加2.4倍。开放涂抹变应原后表皮增殖增加6倍。在丙酮预处理后的斑贴试验条件下，观察到非常强烈的大疱反应。我们得出结论，表皮通透性屏障破坏诱导的表皮LC密度增加伴随着过敏性接触性皮炎反应增强。
