用于定量检测血浆中1型人类免疫缺陷病毒RNA的逆转录酶聚合酶链反应-酶联免疫吸附测定法的开发：与商业定量检测法的比较。
Development of a reverse transcriptase PCR-enzyme-linked immunosorbent assay for quantification of human immunodeficiency virus type 1 RNA in plasma: comparison with commercial quantitative assays.
作者信息
Trabaud M A, Audoly G, Leriche K, Cotte L, Ritter J, Sepetjan M, Trepo C
机构信息
INSERM U271, Lyon, France.
出版信息
J Clin Microbiol. 1997 May;35(5):1251-4. doi: 10.1128/jcm.35.5.1251-1254.1997.
Abstract
A quantitative reverse transcriptase PCR assay with automated detection by nonradioactive hybridization was developed for the determination of human immunodeficiency virus (HIV) type 1 RNA levels. This assay is based on the use of an external standard curve with an internal standard. The accuracy of quantification was verified by comparison with reference commercial tests, the Chiron branched-DNA and Roche AMPLICOR HIV MONITOR assays. This assay was able to quantify viremia in patients with CD4 cell numbers below and above 500/mm3 and to quantify some HIV strains which could not be titrated by the MONITOR assay.
摘要
开发了一种通过非放射性杂交进行自动检测的定量逆转录酶聚合酶链反应检测方法，用于测定1型人类免疫缺陷病毒（HIV）RNA水平。该检测方法基于使用带有内标的外标曲线。通过与参考商业检测方法（Chiron分支DNA检测法和罗氏AMPLICOR HIV MONITOR检测法）进行比较，验证了定量的准确性。该检测方法能够对CD4细胞数低于和高于500/mm3的患者的病毒血症进行定量，并对一些无法通过MONITOR检测法进行滴定的HIV毒株进行定量。
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J Clin Microbiol. 1996 May;34(5):1058-64. doi: 10.1128/jcm.34.5.1058-1064.1996.
2
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J Clin Microbiol. 1994 Feb;32(2):292-300. doi: 10.1128/jcm.32.2.292-300.1994.
3
High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR.通过竞争性聚合酶链反应确定，在感染的所有阶段血浆中均存在高水平的HIV-1。
Science. 1993 Mar 19;259(5102):1749-54. doi: 10.1126/science.8096089.
4
A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes.一种使用电化学发光（ECL）标记探针的单管定量HIV-1 RNA核酸序列扩增检测法。
J Virol Methods. 1994 Sep;49(2):157-67. doi: 10.1016/0166-0934(94)90040-x.
5
Rapid and precise quantification of HIV-1 RNA in plasma using a branched DNA signal amplification assay.使用分支DNA信号放大测定法对血浆中的HIV-1 RNA进行快速精确的定量分析。
J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Apr 15;8(5):446-54. doi: 10.1097/00042560-199504120-00003.
6
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J Acquir Immune Defic Syndr Hum Retrovirol. 1995;10 Suppl 2:S35-44.
7
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8
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9
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