Leyns L, Bouwmeester T, Kim S H, Piccolo S, De Robertis E M
Department of Biological Chemistry, University of California, Los Angeles 90095-1662, USA.
Cell. 1997 Mar 21;88(6):747-56. doi: 10.1016/s0092-8674(00)81921-2.
Frzb-1 is a secreted protein containing a domain similar to the putative Wnt-binding region of the frizzled family of transmembrane receptors. Frzb-1 is widely expressed in adult mammalian tissues. In the Xenopus gastrula, it is expressed and regulated as a typical Spemann organizer component. Injection of frzb-1 mRNA blocks expression of XMyoD mRNA and leads to embryos with enlarged heads and shortened trunks. Frzb-1 antagonizes the effects of Xwnt-8 ectopic expression in a non-cell-autonomous manner. Cultured cells transfected with a membrane-tethered form of Wnt-1 bind epitope-tagged Frzb-1 in the 10(-10) M range. The results strengthen the view that the Spemann organizer is a source of secreted inhibitory factors.
Frzb-1是一种分泌蛋白，其包含一个与卷曲蛋白家族跨膜受体假定的Wnt结合区域相似的结构域。Frzb-1在成年哺乳动物组织中广泛表达。在非洲爪蟾原肠胚中，它作为典型的施佩曼组织者成分表达并受到调控。注射frzb-1 mRNA会阻断XMyoD mRNA的表达，并导致胚胎头部增大、躯干缩短。Frzb-1以非细胞自主方式拮抗Xwnt-8异位表达的作用。用膜结合形式的Wnt-1转染的培养细胞在10^(-10) M范围内结合表位标记的Frzb-1。这些结果强化了施佩曼组织者是分泌性抑制因子来源的观点。
