The effects of thromboxane B2 and 6-ketoprostaglandin F1alpha on cultured fibroblasts.

The effects of thromboxane B2 and 6-ketoprostaglandin F1alpha on the synthesis of DNA, RNA and hexosamine-containing substances were studied. Thromboxane B2 (1 microgram/ml) added to cultures in the stationary phase caused the initiation of DNA synthesis and cell proliferation in a small proportion of cells. The same amount of thromboxane B2 also increased [3H]uridine incorporation into acid-insoluble fraction by 50% in 24 h, and stimulated the production of hexosamine-containing substances 2.5 fold over the control during the first 6 h. On the other hand, 6-ketoprostaglandin F1alpha was essentially inactive on these indexes except slight stimulation of DNA synthesis.