May S L, May W A, Bourdoux P P, Pino S, Sullivan K M, Maberly G F
Department of International Health, Rollins School of Public Health of Emory University, Atlanta, GA, USA.
Am J Clin Nutr. 1997 May;65(5):1441-5. doi: 10.1093/ajcn/65.5.1441.
The measurement of urinary iodine in population-based surveys provides a biological indicator of the severity of iodine-deficiency disorders. We describe the steps performed to validate a simple, inexpensive, manual urinary iodine acid digestion method, and compare the results using this method with those of other urinary iodine methods. Initially, basic performance characteristics were evaluated: the average recovery of added iodine was 100.4 +/- 8.7% (mean +/- SD), within-assay precision (CV) over the assay range 0-0.95 mumol/L (0-12 micrograms/dL) was < 6%, between-assay precision over the same range was < 12%, and assay sensitivity was 0.05 mumol/L (0.6 microgram/dL). There were no apparent effects on the method by thiocyanate, a known interfering substance. In a comparison with five other methods performed in four different laboratories, samples were collected to test the method performance over a wide range of urinary iodine values (0.04-3.7 mumol/L, or 0.5-47 micrograms/dL). There was a high correlation between all methods and the interpretation of the results was consistent. We conclude that the simple, manual acid digestion method is suitable for urinary iodine analysis.
在基于人群的调查中，尿碘测量可提供碘缺乏病严重程度的生物学指标。我们描述了为验证一种简单、廉价的手动尿碘酸消化法所采取的步骤，并将该方法的结果与其他尿碘检测方法的结果进行比较。最初，对基本性能特征进行评估：添加碘的平均回收率为100.4±8.7%（平均值±标准差），在0 - 0.95 μmol/L（0 - 12 μg/dL）的检测范围内，批内精密度（CV）< 6%，在相同范围内的批间精密度< 12%，检测灵敏度为0.05 μmol/L（0.6 μg/dL）。已知的干扰物质硫氰酸盐对该方法没有明显影响。在四个不同实验室与其他五种方法进行的比较中，收集了样本以测试该方法在广泛的尿碘值范围（0.04 - 3.7 μmol/L，或0.5 - 47 μg/dL）内的性能。所有方法之间具有高度相关性，结果解释一致。我们得出结论，这种简单的手动酸消化法适用于尿碘分析。
