Kurashige S, Akuzawa Y, Endo F
Department of Laboratory Sciences, Gunma University School of Health Sciences, Japan.
Immunopharmacol Immunotoxicol. 1997 May;19(2):175-83. doi: 10.3109/08923979709007657.
ICR mice were treated with a carcinogen, N-butyl-N'-butanolnitrosoamine BBN), every day for 8 consecutive weeks and the effects of oral administration of edible mushrooms on the induction of urinary bladder carcinoma and on the activities of macrophages and lymphocytes were studied. Bladder carcinoma were found in all 10 mice (100%) treated with BBN alone, while we observed carcinoma only in 9 of 17 mice (52.9%), in 7 of 15 mice (46.7%) and 13 of 20 mice (65.0%) treated with Lentinus edodes, Grifola frondosa and Pleurotus ostreatus, respectively. Chemotactic activity of macrophages was suppressed in mice treated with BBN alone but maintained almost the normal level in mice treated with BBN plus Lentinus, Grifola or Pleurotus. Lymphocytes collected from mice treated with BBN plus each mushroom showed almost normal blastogenic response against concanavalin A, although those from mice treated with BBN alone completely retarded their response. Cytotoxic activity of lymphocytes against Yac-1 cells was also maintained at a normal level in mice treated with BBN plus each mushroom. Whereas in mice treated with BBN alone significant depression of NK cell activity occurred. Significantly higher cytotoxic activity against P-815 cells was observed in lymphocytes from mice treated with BBN plus each mushroom than that in lymphocytes from normal mice or mice treated with BBN alone.
将ICR小鼠连续8周每天用致癌物N-丁基-N'-丁醇亚硝胺（BBN）处理，研究口服食用菌对膀胱癌诱导以及巨噬细胞和淋巴细胞活性的影响。单独用BBN处理的所有10只小鼠（100%）均发现膀胱癌，而在用香菇、灰树花和平菇处理的小鼠中，我们分别在17只小鼠中的9只（52.9%）、15只小鼠中的7只（46.7%）和20只小鼠中的13只（65.0%）观察到癌。单独用BBN处理的小鼠中巨噬细胞的趋化活性受到抑制，但在用BBN加香菇、灰树花或平菇处理的小鼠中几乎维持在正常水平。从用BBN加每种蘑菇处理的小鼠中收集的淋巴细胞对伴刀豆球蛋白A显示出几乎正常的增殖反应，尽管单独用BBN处理的小鼠的淋巴细胞完全抑制了其反应。在用BBN加每种蘑菇处理的小鼠中，淋巴细胞对Yac-1细胞的细胞毒性活性也维持在正常水平。而单独用BBN处理的小鼠中NK细胞活性显著降低。在用BBN加每种蘑菇处理的小鼠的淋巴细胞中观察到对P-815细胞的细胞毒性活性明显高于正常小鼠或单独用BBN处理的小鼠的淋巴细胞。
