Adachi M, Matsukura S, Tokunaga H, Kokubu F
First Department of Internal Medicine, University of Showa School of Medicine, Shinagawa-ku, Tokyo, Japan.
Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):307-11. doi: 10.1159/000237584.
Bronchial epithelial cells play an important role in the pathogenesis of some inflammatory diseases of bronchial mucosa. Epithelial-cell-derived cytokines are important in the elucidation of the mechanism by which airway inflammation occurs, especially in respiratory virus infection, because these cells are the primary sites of viral infection. We infected bronchial epithelial cells, NCI-H292, with influenza virus A (H3N2) and examined the concentrations of cytokines, interleukin-6 (IL-6), IL-8 and regulated on activation, normal T cells, expressed and secreted (RANTES), in the culture media of infected cells using the enzyme-linked immunosorbent assay system and gene expression of RANTES on epithelial cells by the reverse-transcriptase-polymerase chain reaction method. We found that significant amounts of IL-6, IL-8 and RANTES were released. RANTES mRNA was also detected in infected bronchial epithelial cells. It is suggested that cytokine production in human bronchial epithelial cells may contribute to the pathogenesis of airway inflammatory disorders.
支气管上皮细胞在某些支气管黏膜炎症性疾病的发病机制中起重要作用。上皮细胞衍生的细胞因子在阐明气道炎症发生机制方面很重要，尤其是在呼吸道病毒感染中，因为这些细胞是病毒感染的主要部位。我们用甲型流感病毒（H3N2）感染支气管上皮细胞NCI-H292，并使用酶联免疫吸附测定系统检测感染细胞培养基中细胞因子白细胞介素-6（IL-6）、IL-8和活化正常T细胞表达和分泌因子（RANTES）的浓度，并用逆转录聚合酶链反应法检测上皮细胞上RANTES的基因表达。我们发现释放了大量的IL-6、IL-8和RANTES。在感染的支气管上皮细胞中也检测到了RANTES mRNA。提示人支气管上皮细胞中细胞因子的产生可能有助于气道炎性疾病的发病机制。
