Klapdor R, Scherer K, Sepehr H, Klöppel G
Endoscopy. 1977 Aug;9(3):166-9. doi: 10.1055/s-0028-1098511.
In 4 piglets the gallbladder was punctured under sonographic control threetimes during a period of 46 days. Each puncture was proved by X-ray controls after application of 2-4 ml of a water soluble contrast medium. One day after the third puncture all animals were killed in order to look for macroscopic or microscopic lesions of the gallbladder, the adjacent liver tissue and the peritoneum resulting from the experimental procedure. The results indicate that the ultrasonically guided puncture of the gallbladder represents a procedure to obtain pure bile samples in the piglet or animals of similar size in a simple and non surgical and, therefore, most physiological manner.
在4头仔猪中，在46天的时间里，在超声引导下对胆囊进行了3次穿刺。每次穿刺在注入2 - 4毫升水溶性造影剂后通过X线检查加以证实。第三次穿刺后一天，处死所有动物，以寻找因实验操作导致的胆囊、邻近肝组织和腹膜的宏观或微观病变。结果表明，超声引导下的胆囊穿刺是一种以简单、非手术且因此最符合生理的方式在仔猪或类似大小动物中获取纯胆汁样本的方法。
