Sailaja Y R, Baskar R, Srinivas Rao C S, Saralakumari D
Department of Biochemistry, Sri Krishnadevaraya University, Anantapur 515 003, Andhra Pradesh, India.
Clin Chim Acta. 2004 Mar;341(1-2):185-92. doi: 10.1016/j.cccn.2003.12.003.
The reticulocyte maturation process is an ideal model for the study of biochemical alterations seen during final stage of erythropoiesis under disease conditions. In this study, determined whether type 2 diabetes has any effect on membrane lipids and protein-bound carbohydrates during the maturation of reticulocytes to erythrocytes.
Lipids (cholesterol and phospholipids) and protein-bound carbohydrates (hexose, hexosamine and sialic acid) were extracted and estimated in plasma, membrane of reticulocytes and erythrocytes from 20 treated but uncontrolled type 2 diabetic volunteers and age matched controls.
Plasma, membranes of reticulocytes and erythrocytes of diabetics showed increase in cholesterol (35.7%, 8.7% and 16.4%); phospholipids (43.4%, 18.8% and 8.2%); hexose (34.1%, 19.3% and 8.2%) and decrease in hexosamine (11.9%, 7.3% and 14.7%); and sialic acid (34.1%, 19.3% and 32.0%) compared to controls. As reticulocytes matured to erythrocytes, cholesterol, phospholipids, hexosamine and sialic acid levels were decreased; C/P ratio and hexose levels were increased in both controls and diabetics. However, these alterations were more intensified in diabetics.
These alterations in diabetic patients may indicate the existence of one or both of the following conditions: acceleration of maturation processes and/or decreased red blood cell life span.
网织红细胞成熟过程是研究疾病状态下红细胞生成终末期生化改变的理想模型。本研究旨在确定2型糖尿病在网织红细胞向红细胞成熟过程中是否对膜脂质和蛋白结合碳水化合物有任何影响。
从20名接受治疗但血糖控制不佳的2型糖尿病志愿者及年龄匹配的对照组中提取并测定血浆、网织红细胞膜和红细胞膜中的脂质（胆固醇和磷脂）以及蛋白结合碳水化合物（己糖、己糖胺和唾液酸）。
与对照组相比，糖尿病患者的血浆、网织红细胞膜和红细胞膜中的胆固醇（分别增加35.7%、8.7%和16.4%）、磷脂（分别增加43.4%、18.8%和8.2%）、己糖（分别增加34.1%、19.3%和8.2%）含量升高，而己糖胺（分别降低11.9%、7.3%和14.7%）和唾液酸（分别降低34.1%、19.3%和32.0%）含量降低。随着网织红细胞成熟为红细胞，对照组和糖尿病患者的胆固醇、磷脂、己糖胺和唾液酸水平均降低；C/P比值和己糖水平均升高。然而，这些改变在糖尿病患者中更为明显。
糖尿病患者的这些改变可能表明存在以下一种或两种情况：成熟过程加速和/或红细胞寿命缩短。
