源自三个F2杂交组合的大鼠基因组连锁图谱。

A linkage map of the rat genome derived from three F2 crosses.

作者信息

Bihoreau M T, Gauguier D, Kato N, Hyne G, Lindpaintner K, Rapp J P, James M R, Lathrop G M

机构信息

Wellcome Trust Centre for Human Genetics, University of Oxford, UK.

出版信息

Genome Res. 1997 May;7(5):434-40. doi: 10.1101/gr.7.5.434.

DOI:10.1101/gr.7.5.434

PMID:9149940

Abstract

We report the construction of a dense linkage map of the rat genome integrating 767 simple sequence length polymorphism markers, combined over three crosses with high rates of polymorphism. F2 populations from WKY x S (n = 159), BN x S (n = 91), and BN x GK (n = 139) were selected and genotyped for combinations of microsatellite markers. The loci define 21 linkage groups corresponding to the 20 rat autosomal chromosomes and the X chromosome. The map spans a genetic length of 1998 cM. This combined linkage map should facilitate the advancement of genetic studies for a wide variety of rat models characterized for complex phenotypes.

摘要

我们报告了大鼠基因组密集连锁图谱的构建，该图谱整合了767个简单序列长度多态性标记，这些标记是在三个具有高多态性率的杂交组合中综合得到的。选择了来自WKY×S（n = 159）、BN×S（n = 91）和BN×GK（n = 139）的F2群体，并对微卫星标记组合进行基因分型。这些位点定义了21个连锁群，对应于20条大鼠常染色体和X染色体。该图谱的遗传长度为1998厘摩。这个综合连锁图谱应有助于推进对多种具有复杂表型特征的大鼠模型的遗传学研究。