Taguchi H, Sanada H, Hara K, Miyoshi I, Hiraki K
J Nutr Sci Vitaminol (Tokyo). 1977;23(4):299-304. doi: 10.3177/jnsv.23.299.
The vitamin B12 levels of cerebrospinal fluid were assayed microbiologically (Lactobacillus leichmannii method) using samples from 44 patients with various neurological disorders, 4 patients with megaloblastic anemia and 34 controls. Twenty-seven controls that did not receive vitamin B12 showed a mean cerebrospinal fluid vitamin B12 level of 21.5 pg/ml (range: 0-60). No decrease in cerebrospinal fluid vitamin B12 level was seen in patients with subacute myelo-optico-neuropathy (SMON). High levels of cerebrospinal fluid vitamin B12 were observed only in the patients receiving long term administration of the vitamin. Intrathecal administration of vitamin B12 caused only a slight increase in serum vitamin B12 level after four hours. The existence of blood brain barrier for vitamin B12 was suggested.
采用莱氏乳杆菌法，对44例患有各种神经系统疾病的患者、4例巨幼细胞贫血患者及34名对照者的脑脊液样本进行了微生物学维生素B12水平检测。27名未接受维生素B12治疗的对照者脑脊液维生素B12水平均值为21.5 pg/ml（范围：0 - 60）。亚急性脊髓视神经病变（SMON）患者脑脊液维生素B12水平未见降低。仅在长期接受维生素治疗的患者中观察到脑脊液维生素B12水平升高。维生素B12鞘内注射4小时后，血清维生素B12水平仅略有升高。提示存在维生素B12的血脑屏障。
