Smárason A K, Allman K G, Young D, Redman C W
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, UK.
Br J Obstet Gynaecol. 1997 May;104(5):538-43. doi: 10.1111/j.1471-0528.1997.tb11528.x.
Nitric oxide released from vascular endothelial cells is a potent vasodilator and inhibits platelet adhesion. It has been suggested that decreased nitric oxide production from dysfunctional endothelial cells is implicated in the pathophysiology of pre-eclampsia. In this study evidence was sought for abnormal production of nitric oxide in pre-eclamptic women.
Blood was collected from 20 women presenting with pre-eclampsia, from 20 matched healthy pregnant controls and from 12 nonpregnant women of childbearing age.
Serum nitrate, the stable end metabolite of nitric oxide, was measured by vanadium III chloride reduction and chemiluminescence.
Sera from women with pre-eclampsia had significantly higher nitrate concentrations (mean 47.4 mumol/L [SD 13.6]) compared with healthy pregnant (mean 31.2 mumol/L [SD 9.14]) and nonpregnant (mean 32.1 mumol/L [SD 10.0]) controls.
These results do not support the hypothesis that decreased endothelial cell nitric oxide production may be important in the pathophysiology of pre-eclampsia. On the contrary, serum nitrate levels are increased which may reflect either increased production of nitric oxide from an unidentified source or decreased elimination through the kidneys.
血管内皮细胞释放的一氧化氮是一种强效血管舒张剂，可抑制血小板黏附。有人提出，功能失调的内皮细胞产生的一氧化氮减少与先兆子痫的病理生理学有关。在本研究中，我们探寻先兆子痫妇女一氧化氮产生异常的证据。
采集了20例先兆子痫妇女、20例匹配的健康孕妇对照以及12例育龄非孕妇的血液。
通过三氯化钒还原和化学发光法测定血清硝酸盐（一氧化氮的稳定终末代谢产物）。
与健康孕妇（平均31.2μmol/L[标准差9.14]）和非孕妇（平均32.1μmol/L[标准差10.0]）对照相比，先兆子痫妇女的血清硝酸盐浓度显著更高（平均47.4μmol/L[标准差13.6]）。
这些结果不支持内皮细胞一氧化氮产生减少可能在先兆子痫病理生理学中起重要作用的假说。相反，血清硝酸盐水平升高，这可能反映了来自不明来源的一氧化氮产生增加或经肾脏清除减少。
