Longoni B, Pryor W A, Marchiafava P
Dipartimento di Fisiologia e Biochimica, Universitá di Pisa, Italy.
Biochem Biophys Res Commun. 1997 Apr 28;233(3):778-80. doi: 10.1006/bbrc.1997.6563.
N-Acetylserotonin (NAS) inhibits the peroxidation of linoleic acid induced by a water-soluble initiator, 2,2'-azobis(2-amidinopropane) (ABAP). Lipid peroxidation was detected by the formation of conjugated dienes, monitored spectrophotometrically at 236 nm. N-Acetylserotonin, at concentrations ranging from 200 nM to 20 microM, reduced the rate of ABAP-initiated lipid peroxidation in a concentration-dependent manner. The results of NAS-inhibited lipid peroxidation are compared to the antioxidant activities of melatonin, vitamin E, and a water-soluble vitamin E analog, Trolox. It is suggested that NAS acts as a physiological antioxidant in retinal photoreceptor cells.
N-乙酰血清素（NAS）可抑制由水溶性引发剂2,2'-偶氮双（2-脒基丙烷）（ABAP）诱导的亚油酸过氧化反应。通过共轭二烯的形成来检测脂质过氧化反应，在236nm处用分光光度法进行监测。N-乙酰血清素在200 nM至20 μM的浓度范围内，以浓度依赖性方式降低了ABAP引发的脂质过氧化反应速率。将NAS抑制脂质过氧化反应的结果与褪黑素、维生素E以及水溶性维生素E类似物Trolox的抗氧化活性进行了比较。研究表明，NAS在视网膜光感受器细胞中作为一种生理抗氧化剂发挥作用。
