Lim L, Gibbins J R
Department of Oral and Maxillofacial Surgery, Westmead Hospital, Sydney, Australia.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1995 Feb;79(2):190-8. doi: 10.1016/s1079-2104(05)80281-1.
A panel of five immunohistochemical markers, MB1, leukocyte common antigen, S-100 protein, smooth muscle specific actin, and factor VIII related antigen, were used to study 20 giant cell lesions. These included eight central giant cell granulomas, nine peripheral giant cell granulomas, and three giant cell tumors of bone. The multinucleated giant cells stained positively with MB1, the mononuclear round cells were positive to leukocyte common antigen and the spindle cells were unreactive to all the markers chosen in all the lesions. The most interesting finding was the staining pattern of the blood vessels to factor VIII related antigen in the giant cell granuloma. The blood vessels on the periphery of the lesions were strongly positive for this antibody. However, reaction product was not evident deeper in the lesion within the aggregations of giant cells. Two other endothelial cell markers, Ulex europaeus 1 lectin and QBend 10 were used to study 10 giant cell lesions and a similar pattern of staining was observed. Transmission electron microscopy was subsequently used to study the ultrastructure of the microvasculature of three peripheral giant cell granulomas, and the findings indicated that the reasons for the differential staining may lie in the differences in the structure of the microcirculation.
使用一组五种免疫组织化学标志物（MB1、白细胞共同抗原、S-100蛋白、平滑肌特异性肌动蛋白和因子VIII相关抗原）对20例巨细胞病变进行研究。这些病变包括8例中央巨细胞肉芽肿、9例周围巨细胞肉芽肿和3例骨巨细胞瘤。多核巨细胞MB1染色呈阳性，单核圆形细胞白细胞共同抗原呈阳性，而梭形细胞在所有病变中对所选的所有标志物均无反应。最有趣的发现是巨细胞肉芽肿中血管对因子VIII相关抗原的染色模式。病变周边的血管对该抗体呈强阳性。然而，在病变深处巨细胞聚集区内反应产物不明显。使用另外两种内皮细胞标志物（欧洲荆豆凝集素1和QBend 10）对10例巨细胞病变进行研究，观察到类似的染色模式。随后使用透射电子显微镜研究了3例周围巨细胞肉芽肿微血管的超微结构，结果表明染色差异的原因可能在于微循环结构的差异。
